Part:BBa_K3831031:Design
Construct B for detection of assembly of BMC
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1135
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 880
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Bidirectional terminators BBa_B0014 are located at the 5' and 3' ends of composite part B to prevent the activation of our genes from a native promoter in the chromosome of B. subtilis and to prevent activation of genes located downstream from the composite part. These terminators are separated from the inducible Pgrac by Spacer_5 and from sfGFP by Spacer_7. The coding sequence is placed downstream of the Pgrac promotor with corresponding ribosomal binding site. PduP tag derived from the Pdu operon of P. thermoglucosidasius is connected to sfGFP by a GGGGS-linker. The translation rate of this fusion protein is predicted to be 9 221.97 by RBS calculator.
Source
Original sequence
References
See all the separate Basic Parts.
CHEN, Xiaoying, Jennica L. ZARO a Wei-Chiang SHEN. Fusion protein linkers: Property, design and functionality. Advanced Drug Delivery Reviews [online]. 2013, 65(10), 1357-1369 [cit. 2021-10-7]. ISSN 0169409X. Dostupné z: doi:10.1016/j.addr.2012.09.039.
Wade Y., Daniel R. A., Leak D. J. 2019. Heterologous Microcompartment Assembly in Bacillaceae: Establishing the Components Necessary for Scaffold Formation. ACS Synth. Biol. 8: 1642-1654.
PÉDELACQ, Jean-Denis, Stéphanie CABANTOUS, Timothy TRAN, Thomas C TERWILLIGER a Geoffrey S WALDO. Engineering and characterization of a superfolder green fluorescent protein. Nature Biotechnology [online]. 2006, 24(1), 79-88 [cit. 2021-10-7]. ISSN 1087-0156. Dostupné z: doi:10.1038/nbt1172